Contributions of 5′-UTR and 3′-UTR cis elements to Cyt1Aa synthesis in Bacillus thuringiensis subsp. israelensis
نویسندگان
چکیده
منابع مشابه
Antibacterial activity of Cyt1Aa from Bacillus thuringiensis subsp. israelensis.
Cyt1Aa is a delta-endotoxin protein that is produced by Bacillus thuringiensis subsp. israelensis. It is a membrane pore-forming toxin that is lethal to insect larvae and is broadly cytolytic to vertebrate as well as invertebrate cells. Cyt1Aa is produced as a protoxin of 27 kDa. Proteolytic activation results in a reduction of the molecular mass to approximately 23-24 kDa and a threefold incre...
متن کاملBacillus thuringiensis subsp. israelensis Cyt1Aa synergizes Cry11Aa toxin by functioning as a membrane-bound receptor.
Bacillus thuringiensis subsp. israelensis produces crystal proteins, Cry (4Aa, 4Ba, 10Aa, and 11Aa) and Cyt (1Aa and 2Ba) proteins, toxic to mosquito vectors of human diseases. Cyt1Aa overcomes insect resistance to Cry11Aa and Cry4 toxins and synergizes the toxicity of these toxins. However, the molecular mechanism of synergism remains unsolved. Here, we provide evidence that Cyt1Aa functions a...
متن کاملOral toxicity of Bacillus thuringiensis subsp. israelensis to adult mosquitoes.
The solubilized entomotoxin of Bacillus thuringiensis subsp. israelensis killed adult male and female mosquitoes of several genera and of various physiological states when it was administered orally. Adult mosquito mortality was further influenced when the preparation was contained in sucrose solution. The potential implication for the control of adult mosquitoes is discussed.
متن کاملParasporal body formation via overexpression of the Cry10Aa toxin of Bacillus thuringiensis subsp. israelensis, and Cry10Aa-Cyt1Aa synergism.
Bacillus thuringiensis subsp. israelensis is the most widely used microbial control agent against mosquitoes and blackflies. Its insecticidal success is based on an arsenal of toxins, such as Cry4A, Cry4B, Cry11A, and Cyt1A, harbored in the parasporal crystal of the bacterium. A fifth toxin, Cry10Aa, is synthesized at very low levels; previous attempts to clone and express Cry10Aa were limited,...
متن کاملBacillus thuringiensis subsp. israelensis and Its Dipteran-Specific Toxins
Bacillus thuringiensis subsp. israelensis (Bti) is the first Bacillus thuringiensis to be found and used as an effective biological control agent against larvae of many mosquito and black fly species around the world. Its larvicidal activity resides in four major (of 134, 128, 72 and 27 kDa) and at least two minor (of 78 and 29 kDa) polypeptides encoded respectively by cry4Aa, cry4Ba, cry11Aa, ...
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ژورنال
عنوان ژورنال: Journal of Invertebrate Pathology
سال: 2017
ISSN: 0022-2011
DOI: 10.1016/j.jip.2017.08.002